محیط کشت VRB آگار کد 101406 مرک آلمان





VRB (Violet Red Bile Lactose) agar
acc. ISO 4832 and FDA-BAM GranuCult
Synonym(s): Crystal-violet neutral red bile agar, VRB-agar (Crystal-violet neutral-red bile agar)



Analysis Note

Appearance (clearness): clear
Appearance (colour): red
pH-value (25 °C): 7.2 - 7.6 Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 50 %
Colony colour (Escherichia coli ATCC 8739 (WDCM 00012)): purplish-red colonies with or without precipitate halo
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 50 %
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)): purplish-red colonies with or without precipitate halo
Inoculum on reference medium (Enterobacter cloacae ATCC 13047 (WDCM 00083)):
Colony count (Enterobacter cloacae ATCC 13047 (WDCM 00083)):
Recovery on test medium (Enterobacter cloacae ATCC 13047 (WDCM 00083)): ≥ 50 %
Colony colour (Enterobacter cloacae ATCC 13047 (WDCM 00083)): purplish-red colonies with or without precipitate halo
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): no limit
Colony colour (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): colourless to beige colonies
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Incubation: 24 ± 2 hours at 30 ± 1 °C
Reference media: Tryptic Soy Agar
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.