محیط کشت کروموکالت کولیفرم آگار کد 110426 مرک آلمان





Chromocult Coliform Agar
ISO 9308-1: 2014, For the simultaneous detection and enumeration of coliform bacteria and E. coli
Synonym(s): Coliform Agar, Chromogenic E. coli Agar, Chromogenic Coliform Agar



Analysis Note

Appearance (clearness): slightly turbid to turbid
Appearance (colour): yellowish to yellowish-brown pH-value (25 °C): 6.6 - 7.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)):
Inoculum on reference medium (Enterobacter aerogenes ATCC 13048):
Inoculum on reference medium (Citrobacter freundii ATCC 43864 (WDCM 00006)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Enterobacter aerogenes ATCC 13048):
Colony count (Citrobacter freundii ATCC 43864 (WDCM 00006)):
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 70 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 70 %
Recovery on test medium (Enterobacter aerogenes ATCC 13048): ≥ 70 %
Recovery on test medium (Citrobacter freundii ATCC 43864 (WDCM 00006)): ≥ 70 %
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): partial inhibition
Growth (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024)): no limit
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): dark-blue to violet
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): dark-blue to violet
Colonies (Enterobacter aerogenes ATCC 13048 (WDCM 00175)): pink to red
Colonies (Citrobacter freundii ATCC 43864 (WDCM 00006)): pink to red
Colonies (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024)): colourless
Incubation: 21 ± 3 hours at 36 ± 2 °C
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar