محیط کشت برین هارت براث کد 110493 مرک آلمان




GranuCult Brain Heart Infusion broth 
ISO 6888, To support the growth of fastidious and non-fastidious microorganisms



Analysis Note

Appearance (colour): brown
Appearance (clearness): clear
pH-value (25 °C): 7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 100
Inoculum on reference medium (Streptococcus pneumoniae ATCC 6305): ≤ 100
Inoculum on reference medium (Streptococcus pyogenes ATCC 19615): ≤ 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≤ 100
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): ≤ 100
Inoculum on reference medium (Bacteroides fragilis ATCC 25285): ≤ 100
Inoculum on reference medium (Haemophilus influenzae ATCC 33391): ≤ 100
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): good to very good
Growth (Streptococcus pneumoniae ATCC 6305): good to very good
Growth (Streptococcus pyogenes ATCC 19615): good to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): good to very good
Growth (Candida albicans ATCC 10231 (WDCM 00054)): good to very good
Growth (Bacteroides fragilis ATCC 25285): good to very good
Growth (Haemophilus influenzae ATCC 33391): good to very good
Incubation:
24 ± 2 hours at 37 ± 1 °C aerobic
C.albicans 44 ± 4 hours at 37 ± 1 °C aerobic
B.fragilis 44 ± 4 hours at 37 ± 1 °C anaerobic
H.influenza 44 ± 4 hours at 37 ± 1 °C microaerophilic and with addition of X+V-factor.