محیط کشت EE براث کد 105394 مرک آلمان





Granucult Enterobacteriaceae Enrichment Mossel broth
According to ISO 21528; For microbiology
Synonym(s): EE Broth, MOSSEL Enterobacteriaceae enrichment broth, Enterobacteriaceae enrichment broth, MOSSEL Enterobacteriaceae enrichment broth, Enterobacteriaceae enrichment broth



Analysis Note

Appearance (clearness): clear
Appearance (color): green
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum E.coli resp. Salmonella and E.faecalis.
Additionaly monoculture of E.faecalis
Confirmation on VRBD agar resp. Tryptic soy agar
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Growth on VRBD-Agar(Escherichia coli ATCC 8739 (WDCM 00012)): >10 colonies
Growth on VRBD-Agar(Escherichia coli ATCC 25922 (WDCM 00013)): >10 colonies
Growth on VRBD-Agar(Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 colonies
Growth on VRBD-Agar(Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): pink to red colonies with or without precipitation halo
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): pink to red colonies with or without precipitation halo
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): pink to red colonies with or without precipitation halo
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)): pink to red colonies with or without precipitation halo
Incubation: EE broth: 24 ± 2 hours at 37 ± 1 °C aerobic
Confirmation on VRBD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic